PROTRANS HLA SBT

Sequencing

PROTRANS S4

Sequencing Strategy

• Allele - and Allele-Group specific Amplification Primer dried in PCR Strips
• Splitting the DNA in two Haplotypes and Sequencing each Haplotype separately
• No Ambiguities, because the Haplotypes are amplified and sequenced separately
• Only one Mastermix per DNA
• Identical program for Amplification and Sequencing for:
  
  HLA-Class I:     HLA-A, HLA-B, HLA-C
  HLA-Class II:    HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,
                              HLA-DQB1, HLA-DQA1, HLA-DPB1
  
  
• Software generates the Plate Record automatically
• Low volume or high throughput - manually or full automated
• Safety through automation
• Detection and definition of all new HLA-Alleles
• Results in only one Day
• Complete Exons and easy automated analysis of Sequencing files

Haplotype Specific Sequencing

• HLA class I
• HLA class II